解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Driven by the quest for sustainability, recent years have seen a tremendous progress in bio-based production routes from renewable raw materials to commercial goods. Particularly, the production of organic acids has crystallized as a competitive and fast-evolving field, related to the broad applicability of organic ac...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2015.08.022
更新日期:2015-12-01 00:00:00
abstract::Despite their central role in directing some of the most complex biological processes, carbohydrates--nature's other information carrying biopolymer--have been largely ignored as building blocks for synthetic hierarchical assemblies. The non-stoichiometric binding and astronomical diversity characteristic of carbohydr...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2014.11.016
更新日期:2015-08-01 00:00:00
abstract::Gene expression control is critical to increase production of recombinant proteins, fine-tune metabolic pathways and reliably express synthetic pathways. The importance of transcriptional control seems to be most important in eukaryotic systems. In this review, we highlight recent developments in the field of transcri...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2014.12.015
更新日期:2015-08-01 00:00:00
abstract::For eukaryotic cells to function properly, they divide their intracellular space in subcellular compartments, each harboring specific metabolic activities. In recent years, it has become increasingly clear that compartmentalization of metabolic pathways is a prerequisite for certain cellular functions. This has for in...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2014.11.022
更新日期:2015-08-01 00:00:00
abstract::New microbial tools enable detailed quantification and characterization of complex drinking water (DW) microbiomes. Many opportunities exist from source to tap to apply this knowledge toward management of the microbiology. This requires consideration of the microbiome continuum across all phases harboring microbes (pl...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2014.12.014
更新日期:2015-06-01 00:00:00
abstract::Fungal ligninolytic enzymes have broad biotechnological applications. Particularly laccases and certain fungal class II peroxidases from white-rot basidiomycetes are considered in degradation of persistent organic pollutants. Promising processes with reusable immobilized laccases in special reactors have been develope...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2015.03.006
更新日期:2015-06-01 00:00:00
abstract::Saccharomyces cerevisiae has decisive advantages in industrial processes due to its tolerance to alcohols and fermentation conditions. Butanol isomers are considered as suitable fuel substitutes and valuable biomass-derived chemical building blocks. Whereas high production was achieved with bacterial systems, metaboli...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2014.09.004
更新日期:2015-06-01 00:00:00
abstract::We compare a number of different strategies that have been pursued to engineer thermophilic microorganisms for increased ethanol production. Ethanol production from pyruvate can proceed via one of four pathways, which are named by the key pyruvate dissimilating enzyme: pyruvate decarboxylase (PDC), pyruvate dehydrogen...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2015.02.006
更新日期:2015-06-01 00:00:00
abstract::The intestinal microbiota plays a profound role in human health and extensive research has been dedicated to identify microbiota aberrations that are associated with disease. Most of this work has been targeting the large intestine and fecal microbiota, while the small intestine microbiota may also have a profound imp...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2014.09.005
更新日期:2015-04-01 00:00:00
abstract::Water deficit or drought stress is a major limitation to crop production globally. Plant breeders have used a wide range of technologies to successfully breed varieties that perform well under the growth conditions for their target environments but they are always seeking new opportunities to enhance rates of genetic ...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2014.11.027
更新日期:2015-04-01 00:00:00
abstract::Glutathione transferases (GSTs) represent a major group of detoxification enzymes. All plants possess multiple cytosolic GSTs, each of which displays distinct catalytic as well as non-catalytic binding properties. The progress made in recent years in the fields of genomics, proteomics and protein crystallography of GS...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2014.12.024
更新日期:2015-04-01 00:00:00
abstract::The human gut microbiome is composed of an enormous number of microorganisms, generally regarded as commensal bacteria. Without this inherent microbial community, we would be unable to digest plant polysaccharides and would have trouble extracting lipids from our diet. Resident gut bacteria are an important contributo...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2014.10.007
更新日期:2015-04-01 00:00:00
abstract::RNA imaging probes help us investigate how transport and dynamics of RNA contribute to subcellular RNA localization or regulation of gene expression. Out of the plethora of strategies that have been developed to image RNA in living cells, genetically encoded probes are interesting because they can be produced by the c...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2014.07.012
更新日期:2015-02-01 00:00:00
abstract::Since the development of desorption electrospray ionization (DESI), many other ionization methods for ambient and atmospheric pressure mass spectrometry have been developed. Ambient ionization mass spectrometry has now been used for a wide variety of biological applications, including plant science, microbiology, neur...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2014.07.005
更新日期:2015-02-01 00:00:00
abstract::The versatile functionality of microbial biocatalysts offers a promising solution to the growing need to replace conventional, petroleum-derived fuels, chemicals, and materials with sustainable alternatives from renewable biomass. Whereas metabolic pathway engineering and strain optimization have greatly expanded the ...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2014.04.005
更新日期:2014-12-01 00:00:00
abstract::The increase in quality, quantity, and complexity of recombinant products heavily drives the need to predictably engineer model and complex (mammalian) cell systems. However, until recently, limited tools offered the ability to precisely manipulate their genomes, thus impeding the full potential of rational cell line ...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2014.06.005
更新日期:2014-12-01 00:00:00
abstract::Protein biopharmaceuticals are mainly produced in mammalian cells which can perform human-like post-translational modifications crucial to protein function. Subject to high variability, these critical quality attributes should be monitored and controlled during the manufacturing process. However, the large time requir...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2014.06.019
更新日期:2014-12-01 00:00:00
abstract::The selection of an expression platform for recombinant biopharmaceuticals is often centered upon suitable product titers and critical quality attributes, including post-translational modifications. Although notable differences between microbial, yeast, plant, and mammalian host systems exist, recent advances have gre...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2014.06.007
更新日期:2014-12-01 00:00:00
abstract::Microbes have long been adapted for the biosynthetic production of useful compounds. There is increasing demand for the rapid and cheap microbial production of diverse molecules in an industrial setting. Microbes can now be designed and engineered for a particular biosynthetic purpose, thanks to recent developments in...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2014.02.014
更新日期:2014-10-01 00:00:00
abstract::An intense interest has grown in the unique interactions of nucleic acids with metal ions, which lead to the formation of metal-base pairs and the generation of fluorescent nanomaterials. In this review, different types of metal-base pairs, especially those formed from naturally occurring nucleosides, are described wi...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2013.10.013
更新日期:2014-08-01 00:00:00
abstract::Anaerobic digestion is the default process for biological conversion of residue organics to renewable energy and biofuel in the form of methane. However, its scope of application is expanding, due to availability of new technologies, and the emerging drivers of energy and nutrient conservation and recovery. Here, we o...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2014.01.013
更新日期:2014-06-01 00:00:00
abstract::Contamination of the vadose zone with various pollutants is a world-wide problem, and often technical or economic constraints impose remediation without excavation. In situ bioremediation in the vadose zone by bioventing has become a standard remediation technology for light spilled petroleum products. In this review,...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2013.08.018
更新日期:2014-06-01 00:00:00
abstract::Fermentation of syngas is a means through which unutilized organic waste streams can be converted biologically into biofuels and commodity chemicals. Despite recent advances, several issues remain which limit implementation of industrial-scale syngas fermentation processes. At the cellular level, the energy conservati...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2013.12.001
更新日期:2014-06-01 00:00:00
abstract::Fungal bioactive polysaccharides deriving mainly from the Basidiomycetes family (and some from the Ascomycetes) and medicinal mushrooms have been well known and widely used in far Asia as part of traditional diet and medicine, and in the last decades have been the core of intense research for the understanding and the...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2014.01.010
更新日期:2014-04-01 00:00:00
abstract::Adverse environmental conditions pose serious limitations to agricultural production. Classical biotechnological approaches towards increasing abiotic stress tolerance focus on boosting plant endogenous defence mechanisms. However, overexpression of regulatory elements or effectors is usually accompanied by growth han...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2013.09.011
更新日期:2014-04-01 00:00:00
abstract::Quantitative information about metabolic networks has been mainly obtained at the level of metabolite contents, transcript abundance, and enzyme activities. However, the active process of metabolism is represented by the flow of matter through the pathways. These metabolic fluxes can be predicted by Flux Balance Analy...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2014.01.016
更新日期:2014-04-01 00:00:00
abstract::Organ-on-chip systems integrate microfluidic technology and living cells to study human physiology and pathophysiology. These human in vitro models are promising substitutes for animal testing, and their small scale enables precise control of culture conditions and high-throughput experiments, which would not be econo...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2013.08.015
更新日期:2014-02-01 00:00:00
abstract::The demand for validated analytical methods for botanicals has grown in response to the increasing consumer market for botanical supplements. Government initiatives to increase the availability of validated analytical methods and botanical reference material have led to the publication of numerous validation studies i...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2013.10.003
更新日期:2014-02-01 00:00:00
abstract::Chinese hamster ovary (CHO) cells are the primary factories for biopharmaceuticals because of their capacity to correctly fold and post-translationally modify recombinant proteins compatible with humans. New opportunities are arising to enhance these cell factories, especially since the CHO-K1 cell line was recently s...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2013.02.007
更新日期:2013-12-01 00:00:00
abstract::The gastrointestinal (GI) tract is a complex system characterized by multiple cell types with a determined architectural arrangement. Tissue engineering of the GI tract aims to reinstate the architecture and function of all structural layers. The key point for successful tissue regeneration includes the use of cells/b...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2013.03.021
更新日期:2013-10-01 00:00:00
abstract::Intraportal islet transplantation has emerged as a promising treatment for type 1 diabetes mellitus (T1DM). Nevertheless, long-term efficacy has been limited to a marginal number of patients. Outcomes have been restricted, in part, by challenges associated with the transplant site, poor vascularization, and disruption...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2013.05.004
更新日期:2013-10-01 00:00:00
abstract::Current approaches for treating peripheral nerve injury have resulted in promising, yet insufficient functional recovery compared to the clinical standard of care, autologous nerve grafts. In order to design a construct that can match the regenerative potential of the autograft, all facets of nerve tissue must be inco...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2013.05.006
更新日期:2013-10-01 00:00:00
abstract::Nanopore technology employs a nanoscale hole in an insulating membrane to stochastically sense with high throughput individual biomolecules in solution. The generality of the nanopore detection principle and the ease of single-molecule detection suggest many potential applications of nanopores in biotechnology. Recent...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2012.11.008
更新日期:2013-08-01 00:00:00
abstract::The information encoded in the base sequence of DNA provides substantial structural and functional information for the instructive self-assembly of one-dimensional (1D) functional DNA nanostructures. The hybridization chain reaction (HCR) and the formation of HCR-stimulated DNAzyme nanochains are presented, as a means...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2013.02.005
更新日期:2013-08-01 00:00:00
abstract::Advances in metabolic engineering have given rise to the biological production of novel fuels and chemicals, but yields are often low without significant optimization. One generalizable solution is to create a specialized organelle for the sequestration of engineered metabolic pathways. Bacterial microcompartments are...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2012.11.012
更新日期:2013-08-01 00:00:00
abstract::The structure of microbial communities is key to their functionality. However, this structure is likely to be influenced by adaptive genetic change in members of the community, which can occur over a matter of days. Changes in community structure can in turn influence the evolutionary trajectories of species within th...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2013.02.022
更新日期:2013-08-01 00:00:00
abstract::A central challenge for medicine is to predict disease risk and treatment outcomes for individuals. But what kind of information should be used to make useful predictions in biology? One important cause of phenotypic variation is of course genetics. However genetic predictions have both practical and fundamental limit...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2013.03.004
更新日期:2013-08-01 00:00:00
abstract::The remediation of polychlorinated biphenyls (PCBs) in soils and sediments remains a particularly difficult problem to solve. The possibility of in situ degradation by microorganisms has been pursued for many years since this approach has the potential to provide a cost-effective and environmentally sustainable altern...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2012.10.004
更新日期:2013-06-01 00:00:00
abstract::Culture-independent approaches, such as next-generation sequencing and microarray-based tools, provide insight into the identity and functional diversity of microbial communities. Although these approaches are potentially powerful tools in understanding microbial structure and function, there are a number of limitatio...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2012.10.020
更新日期:2013-06-01 00:00:00
abstract::Both cyanobacteria and eukaryotic microalgae are promising organisms for sustainable production of bulk products such as food, feed, materials, chemicals and fuels. In this review we will summarize the potential and current biotechnological developments. Cyanobacteria are promising host organisms for the production of...
journal_title:Current opinion in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.copbio.2013.04.004
更新日期:2013-06-01 00:00:00